7 Mistakes You’re Making with Peptide Impurity Analysis (and How to Fix Them)
Molecular / analytical context (quick reference) Analyte class: synthetic peptides (variable sequence; variable MW)Common impurity classes: deletions, insertions, truncations, misincorporations, racemization/epimerization, deamidation, oxidation, adducts (TFA/acetate/salts), aggregates/oligomers, charge variantsPrimary analytical toolchain (typical): RP-HPLC/UPLC (C18/C8) + UV (214 nm) ± CAD/ELSD + (HR)LC–MS ± MS/MS; orthogonal: SEC, IEX/CEX, CE, peptide mapping(Plain-language note: impurity profiling is rarely “one…
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